TABLE 2.
Primers used in this study
| Primer | Sequence (5′→3′)a |
|---|---|
| P211W-F | GATATCGTTCCTCACGTTCCTtggCAATCCTTCGGATTCCTTCAT |
| P211W-R | ATGAAGGAATCCGAAGGATTGccaAGGAACGTGAGGAACGATATC |
| L258F-F | CACCTCTATCCTTGATCACttcAGTTACTTTGATATCAACG |
| L258F-R | CGTTGATATCAAAGTAACTgaaGTGATCAAGGATAGAGGTG |
| L258Y-F | CTTTCACCTCTATCCTTGATCACtacAGTTACTTTGATATCAACGAAGG |
| L258Y-R | CCTTCGTTGATATCAAAGTAACTgtaGTGATCAAGGATAGAGGTGAAAG |
| E265A-F | TGAGTTACTTTGATATCAACgctGGAAGCTGTTTGGCGGCC |
| E265A-R | GGCCGCCAAACAGCTTCCagcGTTGATATCAAAGTAACTCA |
| E265D-F | AGTTACTTTGATATCAACgatGGAAGCTGTTTGGCGGC |
| E265D-R | GCCGCCAAACAGCTTCCatcGTTGATATCAAAGTAACT |
| E265N-F | TGAGTTACTTTGATATCAACaacGGAAGCTGTTTGGCGGCC |
| E265N-R | GGCCGCCAAACAGCTTCCgttGTTGATATCAAAGTAACTCA |
| E265C-F | CACTTGAGTTACTTTGATATCAACtgtGGAAGCTGTTTGGCGGCCGC |
| E265C-R | GCGGCCGCCAAACAGCTTCCacaGTTGATATCAAAGTAACTCAAGTG |
| E265H-F | TGAGTTACTTTGATATCAACcacGGAAGCTGTTTGGCGGCC |
| E265H-R | GGCCGCCAAACAGCTTCCgtgGTTGATATCAAAGTAACTCA |
| E265F-F | CACTTGAGTTACTTTGATATCAACtttGGAAGCTGTTTGGCGGCCGC |
| E265F-R | GCGGCCGCCAAACAGCTTCCaaaGTTGATATCAAAGTAACTCAAGTG |
| E265P-F | CTTGAGTTACTTTGATATCAACcctGGAAGCTGTTTGGCGGCCG |
| E265P-R | CGGCCGCCAAACAGCTTCCaggGTTGATATCAAAGTAACTCAAG |
| E265W-F | CACTTGAGTTACTTTGATATCAACtggGGAAGCTGTTTGGCGGCCGC |
| E265W-R | GCGGCCGCCAAACAGCTTCCccaGTTGATATCAAAGTAACTCAAGTG |
| E265V-F | TGAGTTACTTTGATATCAACgttGGAAGCTGTTTGGCGGCC |
| E265V-R | GGCCGCCAAACAGCTTCCaacGTTGATATCAAAGTAACTCA |
| E265Y-F | AGTTACTTTGATATCAACcaaGGAAGCTGTTTGGCGG |
| E265Y-R | CCGCCAAACAGCTTCCttgGTTGATATCAAAGTAACT |
| S267L-F | CTTGAGTTACTTTGATATCAACGAAGGAttgTGTTTGGCGGCCGCAC |
| S267L-R | GTGCGGCCGCCAAACAcaaTCCTTCGTTGATATCAAAGTAACTCAAG |
| S267T-F | ACTTTGATATCAACGAAGGAaccTGTTTGGCGGCC |
| S267T-R | GGCCGCCAAACAggtTCCTTCGTTGATATCAAAGT |
| S267W-F | AGTTACTTTGATATCAACGAAGGAtggTGTTTGGCGGCCGC |
| S267W-R | GCGGCCGCCAAACAccaTCCTTCGTTGATATCAAAGTAACT |
| S267Y-F | AGTTACTTTGATATCAACGAAGGAtacTGTTTGGCGGCCGC |
| S267Y-R | GCGGCCGCCAAACAgtaTCCTTCGTTGATATCAAAGTAACT |
| E265V/S267W -F | CACTTGAGTTACTTTGATATCAACgttGGAtggTGTTTGGCGGCCGCACT |
| E265V/S267W-R | AGTGCGGCCGCCAAACAccaTCCaacGTTGATATCAAAGTAACTCAAGTG |
| S61C-F | TCCTTGCTTtgtGACACAAATG |
| S61C-R | CATTTGTGTCacaAAGCAAGGA |
| S115C-F | TGGTTTCCTTTCCtgtTATGAGCAAGTTG |
| S115C-R | CAACTTGCTCATAacaGGAAAGGAAACCA |
| E190C-F | GCTTACTATGTTtgtTCTACCGGTA |
| E190C-R | TACCGGTAGAacaAACATAGTAAGC |
| E238C-F | TCTGTACTTCTtgtATTGAAACCAAG |
| E238C-R | CTTGGTTTCAATacaAGAAGTACAGA |
a
Mutation sites are shown in italic lowercase.