FIG 5.

N-glycosylation affects TLP assembly. (A) Isopycnic CsCl gradient used to isolate TLPs from MA104 infected with rsSA11 or rsSA11-Y9P-N18A. The cells were infected with viruses at an MOI of 1. (B) Silver staining of the TLP and DLP fractions from MA104 cells. Predicted proteins (based on their molecular weight) are indicated on the right. (C and D) Isopycnic CsCl gradient and silver staining of rsSA11 and rsSA11-Y9P-N18A from HT29 cells. (E) Transmission electron microscopy images showing HT29 cells infected with rsSA11 (left) or rsSA11-Y9P-N18A (right). Viroplasm is marked as “V,” and viral particles are marked by arrows.