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. 2022 Dec 13;133(3):e161759. doi: 10.1172/JCI161759

Figure 9. Involvement of MYC in the LEENE-LEO1 mechanism.

Figure 9

(A) Co-IP of LEO1 and MYC in ECs. (B) RIP with ECs infected with Ad-GFP or Ad-LEENE using anti-MYC antibody or IgG control. LEENE RNA in the immunoprecipitates was quantified by qPCR and the relative enrichment in the Ad-GFP sample was set to 1. (C) qPCR analysis of ECs transfected with scramble or MYC siRNA (siMYC) and infected with Ad-GFP or Ad-LEENE. Bar graphs represent mean ± SEM. ##P = 0.01; **P < 0.01; ###P = 0.001; ***P < 0.001 based on 1-way ANOVA followed by Dunnett’s test. (D) Schematic illustration of LEENE-regulated angiogenic and ischemic responses. LEENE, potentially by binding LEO1 and MYC, promotes the transcription of proangiogenic genes, e.g., those encoding eNOS (NOS3) and VEGFR2 (KDR), to enhance angiogenesis and flow perfusion. Such mechanism is suppressed in diabetic conditions, which contributes to the reduced tissue perfusion in PAD.