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. 2023 Feb 1;133(3):e158705. doi: 10.1172/JCI158705

Figure 4. FAM117B competes with NRF2 for KEAP1 binding in gastric cancer cells.

Figure 4

(A and B) NRF2 protein levels in HGC-27 and AGS cells expressing indicated shRNAs (n = 3 independent experiments). (C) Immunofluorescence assay (n = 3 independent experiments). Scale bars: 10 μm. (D and E) Binding of endogenous FAM117B to KEAP1 in HGC-27 and AGS cells (n = 3 independent experiments). (F) Binding of exogenous HA-FAM117B-WT to FLAG-KEAP1-WT in HEK293T cells (n = 3 independent experiments). (G) Binding of purified GST-KEAP1-WT to purified FAM117B (n = 3 independent experiments). (H and I) Binding levels of FAM117B or NRF2 to KEAP1 in HGC-27 and AGS cells with FAM117B knockdown or overexpression (n = 3 independent experiments). ***P < 0.001, significant difference vs. shControl or Vector group. Statistical significance was calculated using 2-tailed unpaired Student’s t test (2 groups) or 1-way ANOVA followed by Tukey’s test (more than 2 groups).