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. 2023 Feb 1;133(3):e158705. doi: 10.1172/JCI158705

Figure 5. FAM117B binds to the DGR domain of KEAP1 through its ETGE motif.

Figure 5

(A) Diagram of WT and truncated mutants of KEAP1. (B) Binding of exogenous HA-FAM117B-WT to FLAG-KEAP1-WT or truncated mutants in HEK293T cells (n = 3 independent experiments). (C) ETGE motif of FAM117B in different species. (D–G) Interaction of the ETGE motifs of NRF2 and FAM117B with crystal structure of KEAP1-DGR. (H) Diagram of WT and mutants of FAM117B. (I) Binding of exogenous FLAG-KEAP1-DGR to HA-FAM117B-WT or mutants in HEK293T cells (n = 3 independent experiments).