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. Author manuscript; available in PMC: 2023 Jan 31.
Published in final edited form as: Arch Biochem Biophys. 2022 Nov 25;733:109472. doi: 10.1016/j.abb.2022.109472

Table 1.

Steady-State kinetic parameters for WT h12-LOX and Y649C at three temperatures. Standard deviation errors are shown in parentheses from 3 replicates.

 
 kcat (sec−1)
 kcat/KM (μM−1sec−1)
Temperature (°C) WT Y649C WT Y649C
22 22 (4) 2.6 (6) 10 (0.2) 0.76 (0.4)
37 21 (1) 3.6 (0.1) 8.7 (2) 6 (0.6)
42 16 (0.6) 12 (0.3) 16(3) 2.6 (0.2)