FIG. 3.

Cytokine and chemokine production by neurons. Neurons were treated as indicated with IFN-γ and/or TNF for 12 h before RH toxoplasms were added at an MOI of 1. At 48 h after infection the supernatant was harvested, and IL-1β, IL-6, IL-10, GM-CSF, TGF-β1, TGF-β2, MIP-1α, and MIP-1β contents were determined by enzyme-linked immunosorbent assays. IL-10 and GM-CSF were not detected (data not shown). The data are means ± standard deviations based on three wells per group. A second experiment yielded comparable results.