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. 2023 Jan 20;12:e80135. doi: 10.7554/eLife.80135

Figure 10. Confocal micrographs of E-cadherin and claudin 3 immunofluorescence staining from wildtype (WT) and respective knockout (KO) cell lines.

Figure 10.

(A) Localization of E-cadherin and claudin 3 in WT cells, where E-cadherin is distributed over the basolateral plasma membrane and claudin 3 is enriched at apical domains, but can also be found at basolateral membrane regions. (B–H) ARHGAP33-, TM9SF4-, PIP5K1C-, MTMR2-, GALNT2-, TMEM110-, and ANO8-KO cell lines show basolateral E-cadherin localization as well as claudin 3 enrichment at apical and lateral domains, similar to WT cells (A). Scale = 5 µm. (I) TEER measurements of WT and respective KO clones. TEER of ARHGAP33-KO cells increases substantially around day 7 of the measurement. Measurements are depicted as means with standard deviation. XY = top view of polarized monolayer; XZ/YZ = lateral view of polarized monolayer; scale = 5 µm.