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. 2023 Jan 20;12:e80135. doi: 10.7554/eLife.80135

Figure 13. Simplified scheme of the phenotypes observed upon knockout (KO) of ARHGAP33, TM9SF4, PIP5K1C, MTMR2, GALNT2, ANO8, and TMEM110.

Figure 13.

(A) Scheme of a healthy enterocyte. The investigated factors for screen validation and further phenotypic characterization are displayed together with their associated gene ontology (GO) terms. (B) KOs of ARHGAP33, TM9SF4, PIP5K1C, MTMR2, GALNT2, ANO8, and TMEM110 lead to the formation of microvillus inclusions and lateral pseudo-apical domains with microvilli. KO of ARHGAP33, TM9SF4, and ANO8 leads to the formation of enlarged late endosomal/lysosomal compartments positive for Lamp1 and cathepsin D and that contain the apical markers DPP4 and stx3. All KOs additionally lead to aberrant, 'tipi-like' assemblies of apical microvilli.