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. 2023 Jan 20;12:e80135. doi: 10.7554/eLife.80135

Figure 9. Confocal micrographs of syntaxin-3 (stx3) and actin immunofluorescence staining from wildtype (WT) and respective knockout (KO) cell lines.

Figure 9.

(A) stx3 localizes strictly to the apical plasma membrane in WT cells. (B) stx3 abberantly localizes to a subapical compartment in TM9SF4-KO cells. (C) ANO8-KO cell lines display apical localization of stx3. (D) stx3 mislocalizes to large, basolateral, actin-rich compartments in PIP5K1C-KO cells, reminiscent of microvillus inclusions. (E) stx3 localizes to subapical compartments in ARHGAP33-KO cells. (F) stx3 mislocalizes to large, basolateral actin-rich compartments in MTMR2-KO cells. (G, H) GALNT2- and TMEM110-KO cell lines display apical localization of stx3. XY = top view of polarized monolayer; XZ/YZ = lateral view of polarized monolayer. (I) Delocalized stx3 in polarized CaCo2 cells was quantified (dot box plot, Mann–Whitney U test. **p<0.01, ***p<0.005, n.s., not significant, n ≥ 100 cells per condition). XY = top view of polarized monolayer; XZ/YZ = lateral view of polarized monolayer. Scale = 5 µm.