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. 2023 Jan 24;2023:8258354. doi: 10.1155/2023/8258354

Figure 2.

Figure 2

Effects of pterostilbene on intestinal permeability, jejunal apoptosis index, jejunal morphology and the expression of jejunal tight junction proteins in the diquat-challenged broilers. (a) Plasma DAO activity. (b) Plasma D-lactate level. (c) TUNEL analysis was used to identify the apoptotic cells in the jejunum. (d) The quantification of jejunal TUNEL-positive cells. (e) Jejunal villus height. (f) Jejunal crypt depth. (g) The ratio of villus height to crypt depth in the jejunum. (h–j) Western blot analysis was conducted to determine the protein expression of OCLN and ZO-1 in the jejunum. (k, l) qRT-PCR analysis was performed to detect the mRNA expression of OCLN and ZO-1 in the jejunum. Data are shown as mean ± standard error, n = 6/group; ∗P < 0.05 and ∗∗P < 0.01.