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. 2022 Oct 17;19(2):159–167. doi: 10.1038/s41589-022-01147-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — Gel-based ABPP with the BOCILLIN-FL probe labels known PBPs in E. coli NC101 (top left), L. rhamnosus (top right), E. faecalis (bottom left), and K. oxytoca (bottom right) lysates, and inhibitor 3 at concentrations of 10 nM to 500 µM does not inhibit labeling of these proteins. Experiments were conducted twice with similar results.