Fig. 2. Compounds 1–4 inhibit ClbP activity.
a, ClbP activity in vitro upon treatment with 1–4 using a fluorescent substrate analog. b, ClbP activity in vitro upon treatment with 1–4 at 100 nM and varying incubation time before initiating the assay. c, Activity of E. coli overexpressing ClbP toward a fluorescent substrate analog after treatment with 1–4. For panels a–c, symbols show mean value of n = 4 biological replicates for each condition, error bars are 1 s.d. d, LC–MS measurement of N-myristoyl-d-asparagine released from BWpks after treatment with vehicle or 1–4. n = 3 biological replicates. ****P < 0.0001; not significant (NS), P > 0.05, one-way ANOVA and Dunnett’s multiple comparison test. See Methods for calculation of percent activity in fluorescence assays.