Extended Data Fig. 1. Pinacol ester hydrolysis is not the rate-limiting step of ClbP inhibition.
Compounds 1-4 show typical ‘slow binding kinetics’ profiles, with potency increasing with longer incubation times and reaching a maximum at approximately 1 hour. When the inhibitors were presoaked in aqueous buffer, no change to this slow binding behavior is observed, indicating that this behavior is not a result of slow hydrolysis of the pinacol boronic ester, but of interaction with ClbP. Each condition was tested in n = 3 biological replicates. Symbols represent the mean of 3 replicates, error bars represent 1 standard deviation (s.d.).