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. 2022 Jul 15;44(2):381–392. doi: 10.1038/s41401-022-00946-y

Fig. 2. 14-3-3ζ was efficiently knocked down or overexpressed in HK-2 cells.

Fig. 2

a The construction of the 14-3-3ζ (Homo) plasmid was performed by GeneCopoeia. b, c YWHAZ was knocked down and overexpressed at the mRNA level in HK-2 cells. *P < 0.05 and ***P < 0.001 versus the sham group, n = 3. d–g After 14-3-3ζ knockdown, the expression of p-YAP was decreased, and the expression of active YAP was increased. However, the in vitro overexpression of 14-3-3ζ yielded the opposite effect. *P < 0.05, **P < 0.01, and ***P < 0.001 versus the NC group, n = 3. h Co-IP results showed that YAP can combine with 14-3-3ζ in renal tubular epithelial cells.