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. 2022 Nov 19;391(2):393–408. doi: 10.1007/s00441-022-03711-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Schematic diagrams of lineage-tracing mouse models. a Mice expressing Cre under the control of the anti-Müllerian Hormone Type II Receptor (Amhr2) promoter (Amhr2-Cre), were crossed to mice with a loxP-floxed STOP sequence upstream of enhanced yellow fluorescent protein (EYFP) (Rosa26-Stop^fl/fl−EYFP) in the Rosa locus. Upon Cre mediated excision of the STOP sequence, EYFP was constitutively expressed in mesenchymal and mesenchymal-derived cells within the uterus of double transgenic mice (Amhr2-Cre; Rosa26-EYFP). b Amhr2-Cre mice were crossed to mice with a loxP-floxed STOP sequence upstream of the tetracycline trans-activator (tTA) (Rosa26-Stop^fl/fl−tTA), resulting in constitutive expression of tTA. A third cross was made with mice that had a histone H2bj-GFP fusion gene controlled by an up-stream tetracycline-inducible promoter (TRE, tetracycline response element) (TRE-H2b-GFP) resulting in triple transgenic mice (Amhr2-Cre; Rosa26-tTA; H2B-GFP). In Amhr2-Cre; Rosa26-tTA; H2B-GFP mice, tTA bound the TRE, inducing expression of H2B-GFP that was incorporated into nucleosomes serving to label mesenchymal and mesenchymal-derived cells within the uterus