Fig. 3.

MET occurs in the absence of endometrial damage and repair. Representative images of direct YFP fluorescence in uterine cross sections from adult Amhr2-Cre; Rosa-EYFP female mice during proestrus (a, a’’), estrus (b, b’’), metestrus (c, c’’), diestrus (d, d’’), and control mice (no Cre) (e, e’’). EpCAM⁺YFP⁻ (non-MD) and EpCAM⁺YFP⁺ (MD) epithelial cells were analyzed by flow cytometry in proestrus (a’’), estrus (b’’), metestrus (c’’), diestrus (d’’), and control (e’’) with percentages of EpCAM⁺YFP⁺ cells graphed in (f). Percentages of EpCAM⁺YFP.⁺ MD-epithelial cells were compared by estrous cycle stage from virgin and postpartum mice (g). Statistical analyses were performed by one-way ANOVA (f) and t-test for each stage of the estrous cycle (g) with significance at P < 0.05 indicated by different letters. ns, not significant. EpCAM, epithelial cell adhesion molecule; Pro, proestrus; Es, estrus; Met, metestrus; Di, diestrus; Ctrl, control (no cre)