(A) Changes in mature B-cell number in bone marrow (blue) and spleen (red) of mice injected with CFA (i.p.) over 5 d. (B) LTβR ligand expression in mature B cells in bone marrow of mice immunized with CFA for 24 h (Ltb−/−, gray-filled histogram; Ltb+/+, blue histogram). (C, D, E)
Il7-GFP expression Geo. mean in MSCs (C); developing B-cell subsets in bone marrow (D); and inflammatory monocytes and neutrophils in the spleen (E) of control (black) or Cd21-cre Ltbfl/fl mice (purple) injected with CFA i.p. for 5 d. Bars represent the average, and error bars indicate the SEM. *P < 0.05 and **P < 0.005 by an unpaired t test. Data in all panels are representative of two to four experiments.