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. 2023 Jan 30;6(4):e202301924. doi: 10.26508/lsa.202301924

Figure S2. LTβR signaling and TNF receptor signaling control Il7 down-regulation and promote emergency myelopoiesis in response to Listeria.

Figure S2.

(A, B, C) Effects of CFA-induced systemic inflammation in hematopoietic progenitor and myeloid cell proliferation. (A) BrdU incorporation in LSKs, CMPs, GMPs, MEPs, monocytes, and neutrophils on day 3 after CFA (i.p.) of control (black) and LTβR cKO (blue) mice. BrdU injection was 2 h before euthanasia. (B) Number of BrdU+ monocytes and neutrophils in the spleen. Mice were kept under BrdU exposure for 48 h. (C) Total number of monocytes and neutrophils in the spleen. (E, F, G) Total number of developing B cells and myeloid cells in control (black) and LTβR cKO (blue) mice under homeostasis. (D) Number of Lepr+ MSCs in bone marrow. (E) B-cell subsets in bone marrow. (F) Monocytes and neutrophils in bone marrow. (G) Monocytes and neutrophils in the spleen. (H, I, J) Total number of developing B cells and myeloid cells in control (black) and LTβR cKO (blue) mice analyzed 2 d after infection with Listeria. (H) B-cell subsets in bone marrow. (I) Monocytes and neutrophils in bone marrow. (J) Monocytes and neutrophils in the spleen. (K, L, M) Total number of developing B cells and myeloid cells in control (black) and LTβR cKO (blue) mice analyzed 3 d after infection with Listeria. (K) B-cell subsets in bone marrow. (L) Monocytes and neutrophils in bone marrow. (M) Monocytes and neutrophils in the spleen. Bars represent the average, and circles depict individual mice. *P < 0.05 by an unpaired t test.