Figure S3. LTβR signaling and TNF receptor signaling control Il7 down-regulation and promote emergency myelopoiesis in response to the viral mimetic polyI:C, and effects of glucocorticoid receptor deficiency in B-lineage cells in emergency myelopoiesis.

(A, B) Number of developing B-cell subsets in bone marrow (A) and myeloid cell subsets in the spleen (B) of mice immunized with polyI:C i.v. (1.4 mg/Kg/d) for 4 d pretreated with Hel-Ig, LTβR-Ig, or anti-LTβR-Ig + anti-TNF-α. (C, D) Il7-GFP expression in saline versus polyI:C-immunized control or LTβR∆ mice (day 5). (E, F, G) Impact of Ifnar deletion in MSCs in emergency myelopoiesis in response to viral mimetic and susceptibility to HSV2 viral infection. (E) Il7-GFP Geo. mean in Lepr+ MSCs. (F) Number of developing B cells in bone marrow. (G) Monocytes and neutrophils in the spleen. (H, I) Number of developing B-cell subsets in bone marrow (H) and myeloid cell subsets in the spleen (I) of control and Nr3c1^fl/fl Mb1^cre/+ mice immunized with CFA for 5 d. (J, K) Number of developing B-cell subsets in bone marrow (J) and myeloid cell subsets in the spleen (K) of control and Nr3c1^fl/fl Mb1^cre/+ mice immunized with CFA for 5 d and pretreated with HEL-Ig or with LTβR-Ig + anti-TNF-α. (L, M, N) Effects of CFA-induced systemic inflammation in mice conditionally deficient in Ltb in B-lineage cells. (L) Il7-GFP Geo. mean in Lepr+ MSCs in bone marrow. (M) Developing B-cell numbers. (N) Neutrophil and monocyte numbers in the spleen. Mice were Mb1^+/+; Ltb^fl/fl (CTR, black) and Mb1^cre/+; Ltb^fl/fl and challenged with CFA (ip) for 5 d. (O, P, Q) Numbers of developing B cells, monocytes, and neutrophils in mice conditionally deficient in Ltb in B-lineage cells under homeostasis. (O) Il7-GFP Geo. mean in Lepr+ MSCs in bone marrow. (P) Developing B-cell numbers. (Q) Neutrophil and monocyte numbers in the spleen. Bars represent the average, and circles depict individual mice. *P < 0.05; **P < 0.005; and ***P < 0.0005 by an unpaired t test.