Fig. 1.

Taz negatively regulates Hh signaling. A, B Gli-luciferase and western analyses in C3H10T1/2 cells transfected with myc-Taz or Taz siRNA and further cultured with or without N-Shh at 100 ng/ml for 24 (western) or 42 hrs (luciferase). C, D Gli-luciferase and western analyses in C3H10T1/2 cells transfected with myc-Taz or Taz siRNA in combination with vector or YFP-Smo* and further cultured for 24 or 42 hrs. E Gli-luciferase and western analyses in Gli2-knockout C3H10T1/2 cells transfected with myc-Taz in combination with His-ΔNGli2 and further cultured for 24 hrs. F Gli-luciferase and western analyses in C3H10T1/2 cells transfected with Taz siRNA in combination with Gli3 siRNA and further cultured for 42 hrs. G qRT-PCR analyses in Taz^+/+ or Taz^−/− MEFs treated with N-Shh at 100 ng/ml for 48 hrs. Western analyses were either duplicated or triplicated and the mean value was present under the bands. Error bars, SD; * p < 0.05, **^,++ p < 0.01, n = 3