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. 2023 Jan 18;13:1076631. doi: 10.3389/fmicb.2022.1076631

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Copyright © 2023 Shehata, Hassane and Newmaster.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluating the analytical sensitivity of Lacticaseibacillus paracasei 8700:2 strain-specific identification assay. Three DNA dilution series were prepared from starting concentrations of 10 ng/μl (Dilution series 1), 5 ng/μl (Dilution series 2), and 2 ng/μl (Dilution series 3). Dilution series consisted of five points each (10-fold) and each dilution was tested in triplicate to establish standard curves. The limit of detection was determined to be 0.5 pg. of DNA, corresponding to 150 target copies.