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. 2023 Jan 18;13:1076631. doi: 10.3389/fmicb.2022.1076631

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Copyright © 2023 Shehata, Hassane and Newmaster.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optimization of viability treatment of L. paracasei 8700:2 strain-specific quantitative assay. (A) Final concentrations of PMAxx of 0, 25, 50, 100, and 150 μM were evaluated. PMAxx at concentrations of 25, 50, 100, and 150 μM resulted in significant shifts in Cq values from heat killed samples. PMAxx at a final concentration of 50 μM was selected as a potential optimal concentration. (B) Confirming 50 μM as an efficient PMAxx concentration using five mono-strain reference samples.