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Determination of linear dynamic range, and reaction efficiency of L. paracasei 8700:2 strain-specific quantitative assay. Three independent serial dilution series were prepared from L. paracasei 8700:2 reference samples. The linear dynamic range was established between 6*102 to 6*105 genomes. Reaction efficiency was 91.5, 93.7, and 90.9%. A vertical line at a Cq value of 33 cycles refers to reaction cut-off.
