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. 2023 Jan 18;10:1102209. doi: 10.3389/fmolb.2023.1102209

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Copyright © 2023 Kakni, López-Iglesias, Truckenmüller, Habibović and Giselbrecht.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Suspension intestinal organoids show epithelial barrier integrity by a FITC-dextran diffusion assay. Suspension organoids exclude the 4 kDa FITC-dextran, showing tight epithelial barrier formation (top row). When organoids are treated with 2 mM EDTA (bottom row), the barrier is disrupted and FITC-dextran diffuses into the intracellular space and the organoid lumen. White boxes in the middle images delineate the areas shown in the zoomed images to the respective right. Scale bars: 100 μm (left and middle) and 50 μm (right).