FIGURE 6.

Induction and inhibition of CYP3A4 and MDR1. (A, B) Assessment of CYP3A4 (A) and MDR1 (B) levels in organoids grown embedded in Matrigel domes and suspension, following drug treatment with the inducers rifampicin and 1,25-dihydroxyvitamin D3 and the inhibitors verapamil and ivacaftor for 48 h. In both organoid models, there are significant differences in the expression levels following treatments compared to DMSO-treated controls. Notably, the upregulation of MDR1 expression following treatment with rifampicin and 1,25-dihydroxyvitamin D3 was more robust in the apical-out organoids than in the apical-in organoids. Error bars indicate mean ± S.E.M (n = 3).