TABLE 3.

Cut‐off values and out‐of‐bag performance estimates for different target metrics for the datasets for the IMMULITE 2000XPi and Mercodia Equine Insulin ELISA assays

Assay	Timepoint (min)	Cut‐off	Accuracy	Sensitivity	Specificity	Cohen's Kappa
IMMULITE	0	6.69 [5.31‐8.66]	0.81 [0.72‐0.89]	0.74 [0.33‐1]	0.83 [0.73‐0.93]	0.43 [0.15‐0.68]
IMMULITE	60	44.7 [28.4‐72.42]	0.87 [0.76‐0.95]	0.81 [0.5‐1]	0.88 [0.72‐1]	0.58 [0.36‐0.8]
IMMULITE	120	83.41 [70.1‐98.51]	0.88 [0.8‐0.95]	0.82 [0.43‐1]	0.89 [0.79‐0.97]	0.6 [0.36‐0.84]
IMMULITE	180	74.2 [42.94‐106.17]	0.95 [0.86‐1]	0.89 [0.5‐1]	0.96 [0.84‐1]	0.83 [0.56‐1]
Mercodia	0	10.43 [8.63‐12.33]	0.75 [0.63‐0.87]	0.73 [0.43‐1]	0.76 [0.59‐0.92]	0.4 [0.18‐0.64]
Mercodia	60	65.25 [53.8‐81.04]	0.91 [0.83‐0.97]	0.87 [0.67‐1]	0.92 [0.8‐1]	0.74 [0.560.91]
Mercodia	120	92.42 [80.98‐107.55]	0.83 [0.74‐0.91]	0.77 [0.44‐1]	0.85 [0.73‐0.96]	0.55 [0.33‐0.77]
Mercodia	180	62 [50.95‐75.46]	0.89 [0.81‐0.97]	0.85 [0.62‐1]	0.91 [0.77‐1]	0.71 [0.51‐0.9]