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. 2023 Jan 31;6:127. doi: 10.1038/s42003-023-04489-8

Fig. 4. Melanosome aggregation response to Trp channel agonists and antagonists.

Fig. 4

a Pigmentation index for stage 42/43 Xenopus tadpoles treated at 24 °C for 30 min at the indicated concentrations of TRPA1 (AITC) and TRPM8 (menthol; WS12) agonists. Reversibility (Rev) 6 h after removing the WS12. b, c A TRPM8 antagonist (PF05105679) blocks melanosome aggregation induced by cool temperature (6 °C) (b), while the TRPM4 blocker CBA has no effect (c); Each dot represented the measurement in one tadpole, and the bar is the mean with 95% confidence interval. n = 9 embryos; N = 3 independent experiments; **p < 0.01, ***p < 0.001; ****p < 0.0001; ANOVA followed by Bonferroni’s test.