Table 3.

Trp concentrations of different blood sera were determined via HPLC (estimated error of 10% based on deviations within the repetitions) and emission-based microplate reader measurements of rotaxane 1 at 25 °C

Serum sample	[l-Trp] (µM) in serum (HPLC)	Intersect with calibration curve [l-Trp] (µM) in serum (microplate reader)	Concentration range [l-Trp] (µM) in serum (microplate reader)
Steroid-depleted human serum	<1.0 ± 0.1	2.2	0.6–2.8
Human serum 1	18.3 ± 0.2	16.6	15.1–19.1
Human serum 2	21.9 ± 0.2	18.8	16.1–19.5
Bovine calf serum	38.2 ± 0.4	37.2	32.9–39.6