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. 2023 Jan 31;13:1711. doi: 10.1038/s41598-023-28635-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of three chemotherapy agents on cell viability/death and RNA integrity. A2780 cells were treated with different concentrations of doxorubicin (left panels), docetaxel (middle panels) or irinotecan (right panels) for 72 h. (a) RNA disruption assay. Total RNA was isolated from cells, and RNA disruption was quantified using the RDA. (b) Cell counting assay. Total cells were counted using a haemocytometer following drug treatment. Untreated cells were counted prior to treatment (‘0 h’) to provide a baseline count. (c) Recovery assay. Drug-treated cells were collected, washed, resuspended in a drug-free medium and seeded into plates. After 96 h, the culture’s confluence was measured and normalized to its confluence at 2 h post-seeding. (d) DNA content analysis. Drug-treated cells were collected, washed, fixed, and stained with propidium iodide then analyzed by flow cytometry. Data are presented as means ± standard deviation, with individual data points shown in red. Groups labelled with an asterisk were significantly greater (panels a and d) or significantly lower (panels b and c) than the ‘0 h’ (panel b) or untreated (panels a, c and d) control. See Supplementary Table S3 for detailed results of each statistical test. n/a, extensive RNA disruption in the sample precluded RDA analysis.