Figure 6.

Origin of RNA disruption products. Total RNA was isolated from A2780 cells treated with doxorubicin (DOX) for 48 h. The RNA was then resolved by denaturing gel electrophoresis and transferred onto a PVDF membrane prior to hybridization with radiolabeled DNA probes specific to the 28S rRNA. UV-visualized, ethidium bromide-stained agarose gel images (left panels) and autoradiograms of the northern blots (right panels) are shown for the respective 28S rRNA probes. Arrows indicate the position of the full-length 28S and 18S rRNA bands. Each gel image and autoradiogram are representative of two independent biological replicates. Gel images and autoradiograms were cropped for clarity and conciseness. Full-length, uncropped gel images and autoradiograms are available in Supplementary Fig. S7.