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. 2023 Jan 31;14:509. doi: 10.1038/s41467-023-36071-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Representative H&E images and annotated regions for two patient samples processed by either RRST (n = 4) or standard Visium (n = 2) protocol. The spots in each tissue section were labeled into three categories: mucosa, submucosa, and muscularis. b Distribution of UMI counts in the tissue sections shown in (a). The color scale represents log10-transformed counts. c Distribution of unique genes per spot in the three annotated regions (mucosa, submucosa, and muscularis) visualized as violin plots, for all tissue sections. The y-axis shows log10-transformed counts. d Distribution of UMI counts per spot in the three annotated regions (mucosa, submucosa, and muscularis) visualized as violin plots. The y-axis shows log10-transformed counts. e Expression of 11 epithelial markers in the mucosa for the two adult colon samples visualized as violin plots. A comparison between the two protocols is shown for each gene and the corresponding detection rate is highlighted below each violin plot. The detection rate is defined as the percentage of spots (in the mucosa) where the gene is detected.