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. 2023 Jan 31;13:1728. doi: 10.1038/s41598-023-28165-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) All groups showed spatial habituation learning and moved less on day 2 than day 1. *p < 0.001). (B) There was a day x sex interaction for time spent in the center of the open field (F^1,35 = 4.998, p = 0.032). In females, there was an effect of day with females spending less time spent in the center of the open field on day 2 than day 1. ^op = 0.003. (C) In the open field containing objects, E3-HL mice moved more than E3-TR mice. *p < 0.001. (D) Object recognition was comparable in all groups. The behavioral testing of the mice was performed at 7 months of age.