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. 2023 Jan 31;13:1728. doi: 10.1038/s41598-023-28165-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Overlap of raw chromatograms of human plasma isolated lipoproteins VLDL, LDL and HDL. (B,C) Raw chromatograms of plasma from female and male E3-HL (B) and E3-TR mice (C). (D,E) ApoE elution profile in the six fraction pools obtained after SEC fractionation of plasma from female and male E3-HL (D) or E3-TR mice (E). Plasma Pool 1: Fractions 1–4 containing molecules larger than VLDL, Pool 2: Fractions 5–12 corresponding to the VLDL eluted fraction, Pool 3: Fractions 13–19 corresponding to the LDL eluted fraction, Pool 4: Fractions 20–22 corresponding to HDL eluted fraction, Pool 5: Fractions 23–30 corresponding to fractions with molecular size molecules ranging between 99 and 5 kDa, Pool 6: Fractions 31–60 corresponding to fractions with lower than 5 kDa proteins.