Figure 4.

SUMO inhibition remodels the T-cell compartment towards a memory and activated T-cell phenotype. (A) Experimental setup as outlined in Figure 3A. Pie charts representing the frequencies of the indicated cell populations in the CD3⁺CD4⁺ bone marrow (BM) and spleen compartment (CD3⁺CD4⁺CD44^lowCD62L⁺ naïve T cells, CD3⁺CD4⁺CD44^highCD62L^- memory T cells, CD3⁺CD4⁺CD44^lowCD62L^- effector T cells, CD3⁺CD4⁺CD25⁺CD69⁺FoxP3⁺ regulatory T cells). (B) Percentage of memory T cells (CD3⁺CD4⁺CD44^highCD62L^-) of the CD3⁺CD4⁺ BM and spleen cells. N=6, P values were determined by unpaired t-test. (C) Pie charts representing the frequencies of the indicated cell populations in the CD3⁺CD8⁺ BM and spleen compartment (CD3⁺CD8⁺CD44^lowCD62L⁺ naïve T cells, CD3⁺CD8⁺ CD44^highCD62L^- effector memory T cells, CD3⁺CD8⁺CD44^highCD62L⁺ central memory T cells, CD3⁺CD8⁺CD25⁺CD69⁺ activated T cells) after carrier vs. SUMOi treatment. (D) Percentage of activated T cells (CD3⁺CD8⁺CD25⁺CD69⁺) of the CD3⁺CD8⁺ BM and spleen cells. N=6, P values were determined by unpaired t-test.