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. 2023 Jan 18;11:1103923. doi: 10.3389/fcell.2023.1103923

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Copyright © 2023 Li, Luo, Li, Liu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RNA-seq analysis of dIlp8 mutant fly. (A) Volcano plot of differently expressed genes in dIlp8 ^−/− virgin females. A total of 667 genes were differentially expressed, among which 410 genes were downregulated (green dots) and 257 genes were upregulated (red dots). (B) The mRNA levels of representative DEGs were examined by qRT-PCR (red columns) and compared with the RNA-seq data (blue columns). (C) Heatmap of the innate immune signaling pathway. (D) Heatmap of genes with known roles in female fertility. (E) Heatmap of genes involved in steroid hormones biosynthesis. (F) Heatmap of metabolism genes. The expression level of all genes was normalized by zero-to-one method.