Figure 3.

Bd infection alters hellbender skin gene expression at both (a) early and (b) late infection. Principal components analysis of DEGs identified in pairwise comparisons of high-dose Bd-infected (n = 12) and sham Bd-infected control (n = 6) hellbenders. Each plot represents gene expression comparisons between pre-vaccination (day 0) and either early infection (day 117, (a)) or late infection (day 187, (b)). Expression values are measured as Log₂ transformed counts per million transcripts. Ellipses indicate 95% CI of expression values by Bd status: Bd (−) includes all animals at pre-vaccination and control animals at both early and late infection, Bd (+) includes high-dose Bd-infected animals at both early and late infection.