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. 2022 Dec 12;22(2):274–286. doi: 10.1158/1535-7163.MCT-21-0903

Figure 5.

Figure 5. Converted cells exhibit an increase in the ATP-induced response. A, Pseudocolored Fluo4 fluorescence images from U87-B9 and U3054-A cells. Scale bar = 50 μm. B, Representative ΔF/F0 traces showing the intracellular calcium activity upon ATP stimulation in responsive cells. C, Traces of Fluo4 fluorescence changes in U87 and U3054 cells stimulated by 100 μmol/L ATP administration. Average traces are represented as solid lines. Shaded areas indicate the standard deviation of the mean. D, Quantification of the percentage of cells showing ATP response signals, percentage of cells with oscillatory behavior of such responsive population, and quantification of the peak amplitude of the ATP-evoked calcium elevations. n = 5 independent experiments for each experimental condition. *, P < 0.05; **, P <  0.01; ***, P <  0.001. Bar graphs show mean frequency (±SEM).

Converted cells exhibit an increase in the ATP-induced response. A, Pseudocolored Fluo4 fluorescence images from U87-B9 and U3054-A cells. Scale bar, 50 μm. B, Representative ΔF/F0 traces showing the intracellular calcium activity upon ATP stimulation in responsive cells. C, Traces of Fluo4 fluorescence changes in U87 and U3054 cells stimulated by 100 μmol/L ATP administration. Average traces are represented as solid lines. Shaded areas indicate the standard deviation of the mean. D, Quantification of the percentage of cells showing ATP response signals, percentage of cells with oscillatory behavior of such responsive population, and quantification of the peak amplitude of the ATP-evoked calcium elevations. n = 5 independent experiments for each experimental condition. *, P < 0.05; **, P <  0.01; ***, P <  0.001. Bar graphs show mean frequency (± SEM).