Skip to main content
. 2023 Jan 18;12:1101901. doi: 10.3389/fonc.2022.1101901

Figure 1.

Figure 1

Extracellular matrix of lung and lymph nodes obtained by decellularization. (A) Representative H&E stainings of lung and lymph node before (T=0) and after (T=decell) decellularization show efficiently removal of cells from the scaffold and maintenance of ECM structure. Scale bars indicate 200 µm. 1 and 2 show different donors for dLU (dLu2, dLu3) and dLN (dLN2, dLN12). (B) Representative DAPI stainings of lung (dLu2) and lymph node (dLN6) before and after decellularization confirmed removal of nuclear material from the scaffold. Scale bars indicate 250 µm. (C) Lung slices of 400 µm thick and an entire lymph node of 0.75 cm thick before and after decellularization, show the transformation in color from brown/yellow to translucent white. (D) Quantitative DNA content analysis of lung (n=3 patients, with each patient measured in technical duplicate, p=0.003) and lymph node (n=13, p=0.0002) before and after decellularization confirms successful decellularization. Error bars indicate ± SD. ** = p-value < 0.005, *** = p-value < 0.001. Paired t-tests were used for determining significance in DNA content. The red dotted line indicates a threshold of 50 ng DNA/mg wet tissue, which is a common criteria for adequate cell removal (29). For the DNA content dLu1-3 and dLN1-13 were used. .