Figure EV2. Purification and characterization of ABCA7 and ABCA7_AAA .

1. SEC chromatograms and SDS‐PAGE analysis of ABCA7 and ABCA7_AAA in DDM/CHS.
2. SDS‐PAGE gel and ATPase activity comparison of ABCA7 and ABCA7_AAA reconstituted in liposomes. Experimental replicates (n) = 6 and error bars represent standard deviation (s.d.). Statistical significance by unpaired, two‐tailed t‐test P‐value of < 0.0001 is indicated by ****.
3. Detection of endogenous lipids co‐purified with ABCA7 and ABCA7_AAA by thin‐layer chromatography (TLC).

Source data are available online for this figure.