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. 2022 Nov 30;64(6):1199–1214. doi: 10.5187/jast.2022.e60

Fig. 8. Effect of knock-down APOH gene on the expression of lipid metabolism-related proteins in CS2s (n=3).

Fig. 8.

(a) Western blot showing the protein expression of lipid metabolism-related genes after transfection with pGPU6-GFP-Neo-APOH and pGPU6-GFP-Neo. (b) Relative folds of protein levels (proteins/GAPDH) from the western blots in (a) were quantified by a greyscale scan. (c) pAKT/AKT ratios from the western blots in (a) were quantified by a greyscale scan. (d) pAMPK/AMPK ratios from the western blots in (a) were quantified by a greyscale scan. Data are the mean ± SD from three experiments, nss p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. CS2s, duck myoblasts; AKT1, AKT serine/threonine kinase 1; AMPK, AMP-activated catalytic subunit alpha 1; ACC1, acetyl-CoA carboxylase 1; PPARG, peroxisome proliferator activated receptor gamma; LPL, lipoprotein lipase; ACSL1, acyl-CoA synthetase long chain family member 1; ELOVL6, ELOVL fatty acid elongase 6.