Table 1. List of primer sequences used for qRT-PCR.
| Genes | Primer sequences (5’-3’) | Product (bp) | Temperature (°C) | |
|---|---|---|---|---|
| APOH | F | AAAAGTCTGTTCCAAGCCACC | 249 | 58.5 |
| R | GGCACAGGAGCGTGTTGAG | |||
| AKT1 | F | AGGTTAGGTGGCGGTCCTGATG | 225 | 60 |
| R | GCGGTTCCACTGGCTGAATAGG | |||
| AMPK | F | CGACAGAAGATTCGCAGCCTTG | 232 | 52 |
| R | TGTGACAGTAATCCACGCCAGA | |||
| ACC1 | F | GGTCCTCCAAGCCAAGCAATGT | 201 | 57 |
| R | CGAAGACCACTGCCACTCCAAG | |||
| PPARG | F | GCCACAAGCGGAGAAGGAGAAG | 186 | 53 |
| R | GCAGCGGTGACACATGCTTACA | |||
| ACSL1 | F | CGTTACTCCACCGAGGCTTCAA | 115 | 56 |
| R | TCATAGAGCGGCACCACTACCA | |||
| ELOVL6 | F | CAGTCAGTGTGCGACCAGAGTT | 226 | 60 |
| R | CCAGCCACCATGTCCTTGTAGG | |||
| FAS | F | CCTACTAAGCAGCCTGAGAATG | 130 | 55 |
| R | AGATTGTCCGCCTTCCTGAT | |||
| LPL | F | CCAAGACCAACCAGCCATTCCT | 243 | 60 |
| R | CGCCTGACTTCACTCTGACTCT | |||
| GAPDH | F | GGAGAAACCAGCCAAGTAT | 177 | 60 |
| R | CCATTGAAGTCACAGGAGA | |||
1)
qRT-PCR, quantitative real-time polymerase chain reaction; bp, base pair; APOH, apolipoprotein H; AKT1, AKT serine/threonine kinase 1; AMPK, AMP-activated catalytic subunit alpha 1; ACC1, acetyl-CoA carboxylase 1; PPARG, peroxisome proliferator activated receptor gamma; ACSL1, acyl-CoA synthetase long chain family member 1; ELOVL6, ELOVL fatty acid elongase 6; FAS, fas cell surface death receptor; LPL, lipoprotein lipase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.