TABLE 20.
Motif characterization exemplified by proteins of the PHS family (family 9)a
| Sequence nameb | Residues comparedc | Motif diagramd | Motif Be | Motif De |
|---|---|---|---|---|
| PT1 Stu (a) | 1–283 | 26-[A]--52--[B]-42-[C]--63--[D]-38 | DKMGRKKVY | MPETARYTALV |
| PT2 Ath (a) | 1–278 | 27-[A]--52--[B]-41-[C]--63--[D]-33 | DKLGRKKVY | MPETARYTALV |
| AtPT2 Ath (a) | 1–269 | 36-[A]--45--[B]-42-[C]--53--[D]-31 | DRLGRKRMY | IPETPRYTMDV |
| GuPT Gve (a) | 1–282 | 36-[A]--45--[B]-42-[C]--53--[D]-44 | DRLGRKRMY | IPETPRYTMDV |
| PT2 Stu (a) | 1–283 | 29-[A]--53--[B]-42-[C]--63--[D]-34 | AKMGRKKVY | MPETARYTALV |
| AtPT1 Ath (a) | 1–281 | 27-[A]--52--[B]-42-[C]--63--[D]-35 | DKLGRKKVY | MPETARYTALV |
| Pho84 Sce (a) | 1–317 | 68-[A]--45--[B]-42-[C]--63--[D]-37 | DIVGRKRIY | IPESPRYQLDV |
| Pho-5+ Ncr (a) | 1–303 | 53-[A]--97---------[C]--63--[D]-37 | DKMWRTVIG | IPETPRYTFDV |
| PT1 Stu (b) | 284–540 | 20-[A′]-54--[B]-41-[C′]-57--[D]-27 | DRIGRFAIQ | VPESKGKSLEE |
| PT2 Ath (b) | 279–523 | 23-[A′]-54--[B]-41-[C′]-57--[D]-12 | DTIGRFKIQ | VPEPKGKSLEE |
| PT2 Stu (b) | 284–527 | 23-[A′]-54--[B]-41-[C′]-57--[D]-11 | DIIGRFAIQ | VPESKGKSLEE |
| Pho84 Sce (b) | 318–587 | 35-[A′]-51--[B]-37-[C′]-54--[D]-35 | DIIGRKPIQ | IPETKRKTLEE |
| AtPT1 Ath (b) | 282–534 | 22-[A′]-54--[B]-41-[C′]-57--[D]-21 | DVIGRFAIQ | VPESKGKSLEE |
| GuPT Gve (b) | 283–521 | 15-[A′]-57--[B]-40-[C′]-48--[D]-21 | DSWGRKPIQ | IPETKGLSLEE |
| AtPT2 Ath (b) | 270–521 | 28-[A′]-57--[B]-40-[C′]-48--[D]-21 | DSWGRKPIQ | IPETKGLSLEE |
| Pho-5+ Ncr (b) | 304–569 | 30-[A′]-53--[B]-37-[C′]-53--[D]-35 | DTVGRKPIQ | IPETKRKTLEE |
| Multilevel consensus sequence | DKIGRKKIQ | IPETKRYTLEE | ||
| L FP Y | V AGKSADV | |||
| M P L |
The MEME and MAST programs (5) were used for motif identification.
Abbreviation of the protein (Table 9) and an indication of whether the N-terminal half (a) or the C-terminal half (b) was examined.
Actual residues in each protein segment analyzed.
Spacing (in numbers of amino acyl residues) between the various motifs detected. These common motifs were as follows: motif A, AGMGFFTDAYDLF; motif A′, SWFLLDIAFY; motif B, DKWGRKKIY; motif C, DYPLSATIMSEYANKKWRGAMMAAVFAMQ; motif C′, NFGPNATTFIVPGEIFPTRYRSTCHGIS; motif D, MPETKRYTLEE.
Motifs B and D as they occur in each of the protein segments examined. The multilevel motif consensus sequences for motifs B and D are provided below the motif B and D sequences, respectively.