FIG. 5.

Overexpression of brlA activates sporulation in submerged culture. The brlA gene was placed under the control of the alcohol-inducible promoter alcA. The alcA(p)::brlA strain and a wild-type strain were grown for 12 h in liquid minimal medium containing glucose to repress brlA expression from the alcA promoter and then shifted to alcA(p)-inducing medium. (B) By 3 h after the medium shift, the alcA(p)::brlA strain produced spores from the tips of hyphae. (A) No conidiation was observed in the wild-type strain even 24 h after the medium shift.