Genes located within the identified gene clusters of endocrocin 5 (Enc), emodin 1 (Emo), geodin 19 (Ged), trypacidin 21 (Tpc), pestheic acid 22 (Pta), cladofulvin 11 (Cla), monodictyphenone 12 (Mdp), the agnestins: e.g.30, 31 (Agn), the cryptosporioptides: e.g.34–36 (Dmx), the secalonic acids from Claviceps purpurea39, 40 (Cpur) and neosartorin 38 (Nsr). Genes on each row are considered likely to encode functionally homologous proteins due to high% protein identity. SDR = short-chain dehydrogenase/reductase.
| PREDICTED FUNCTION | Enc 26 | Emo 55 | Ged 27 | Tpc 28,29 | Pta 30 | Cla 31,32 | Mdp 33 | Agn 34 | Dmx 32 | Cpur 32,35 | Nsr 36 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Steps to emodin | Group V nrPKS | encA | emoG | gedC | tpcC | ptaA | claG | mdpG | agnPKS1 | dmxPKS | 05437 | nsrB |
| MβL-TE | encB | emoB a | gedB | tpcB | ptaB | claF | mdpF | agnL7 | dmxR1 | 05436 | nsrC | |
| Decarboxylase | encD | emoH | gedI | tpcK | ptaD | claH1 | mdpH1 | agnL1 | dmxR15 | 05434 | nsrE | |
| Anthrone oxidase | – | emoM | gedH | tpcL | ptaC | claH | mdpH2 | agnL2 | dmxR16 | 05435 | nsrD | |
| Anth. reductase | Oxidoreductase | – | – | gedF | tpcG | ptaF | claK | mdpK | agnL4 | dmxR7 | 05430 | nsrR |
| Steps to chrysophanol | Reductase | – | – | – | – | – | claC | mdpC | agnL6 | dmxR18 | 05429 | nsrJ |
| Dehydratase | – | – | – | – | – | claB | mdpB | agnL8 | dmxR17 | 05428 | nsrI | |
| RCDO | Ring cleaving dioxygenase | – | – | gedK | tpcI | ptaJ | – | mdpL | agnL3 | dmxR6 | 05427 | nsrF |
| Carboxyl methyltransferase | O-Methyltransferase | – | – | gedG | tpcM | ptaH | – | – | – | – | 05424 | nsrG |
| Grisandiene only | Glutathione S-transferaseb | – | – | gedE | tpcF | – | – | – | – | – | – | |
| (Emodin) O-methyltransferase | – | – | gedA | tpcA | – | – | – | – | – | – | – | |
| Gris. And pes. only | Dihydrogeodin oxidase | – | – | gedJ | tpcJ | ptaE ptaK | – | – | – | – | – | – |
| Dimerase | P450 | – | – | – | – | – | claM | – | – | dmxR5 | 05419 | nsrP |
| C-5 hydroxylase | Monooxygenase | – | – | – | – | – | – | mdpD | – | dmxR9 | 05423 | nsrK |
| C-5S reductase | SDR | – | – | – | – | – | – | – | – | dmxR3 | 05418 | nsrO |
| Xanthone cyclase | SnoAL domain protein | – | – | – | – | ptaG | – | – | agnL5 | dmxR10 | 05417 | nsrS |
| 05425 | nsrQ | |||||||||||
| 05426 | ||||||||||||
| Enc only | 2-Oxoglutarate oxidoreductase | encD | – | – | – | – | – | – | – | – | – | – |
| Geo only | Sulochrin halogenase | – | – | gedL | – | – | – | – | – | – | – | – |
| Tpc only | O-methyltransferase | – | – | – | tpcH | – | – | – | – | – | – | – |
| Pes only | O-methyltransferase | – | – | – | – | ptaI | – | – | – | – | – | – |
| Oxidoreductase | – | – | – | – | ptaL | – | – | – | – | – | – | |
| Isosulochrin halogenase | – | – | – | – | ptaM | – | – | – | – | – | – | |
| Cla only | Oxidoreductase | – | – | – | – | – | claN | – | – | – | – | – |
| Mdp only | Acyl-CoA synthase | – | – | – | – | – | – | mdpI | – | – | – | – |
| Glutathione S-transferaseb | – | – | – | – | – | – | mdpJ | – | – | – | – | |
| Cry only | Hemerythrin-like domain protein | – | – | – | – | – | – | – | – | dmxR11 | – | – |
| SDR | – | – | – | – | – | – | – | – | dmxR12 | – | – | |
| O-Acetyltransferase | – | – | – | – | – | – | – | – | dmxR13 | – | – | |
| Cytochrome P450 | – | – | – | – | – | – | – | – | dmxL3 | – | – | |
| hrPKS | – | – | – | – | – | – | – | – | dmxL2 | – | – | |
| Acyl-CoA carboxylase | – | – | – | – | – | – | – | – | dmxL1 | – | – | |
| SDR | – | – | – | – | – | – | – | – | dmxR8 | – | – | |
| Putative transferase | – | – | – | – | – | – | – | – | dmxR2 | – | – | |
| Sa specific | Hypothetical | – | – | – | – | – | – | – | – | – | 05420 | – |
| Monooxygenase | – | – | – | – | – | – | – | – | – | 05431 | – | |
| SDR (C-5R reductase) | – | – | – | – | – | – | – | – | – | – | – | |
| Neo only | O-Acetlytransferase | – | – | – | – | – | – | – | – | – | – | nsrL |
| Non catalytic | AflR-like transcription factor | – | – | gedR | tpcE | ptaR2 | claE | mdpE | agnL10 | dmxR14 | 05433 | nsrA |
| AflS-like coactivator | – | – | gedD | tpcD | ptaR1 | claA | mdpA | agnL9 | – | 05432 | nsrH | |
| Transcription factor | – | – | – | – | ptaR3 | – | – | agnL11 | – | 05421 | nsrM | |
| MFS transporter | – | 41 650.1 | – | – | – | – | – | agnL12 | dmxR4 | 05422 | nsrN |
a
EmoB was not identified in the original analysis and is missing from the MiBIG database. It was identified during the writing of this review, through softberry analysis of the genomic sequence.
b
Putative glutathione S-transferases are listed in two places (Grisandiene only, and mdp only) due to demonstrating protein identity below 25%, suggesting a lack of functional homology.