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. 2022 Nov 20;56(2):e13363. doi: 10.1111/cpr.13363

FIGURE 4.

FIGURE 4

Knockdown of IL‐22 in MSCsPP by RNA interference. (A) The efficiency of siRNA‐mediated downregulation of IL‐22 was assessed at the RNA level and normalized with respect to the expression of gapdh. (B) Flow cytometry analysis of IL‐22 knockdown at the protein level. Colitis assessments including (C) body weight measurement, (D) disease activity index, (E) evaluation of gross morphology images, (F) colon length measurements, (G) H&E staining and corresponding histological scoring statistical histogram, and (H) inflammatory cytokine expression in CD3+ T cells detected by flow cytometry. Scale bar, 100 μm. Data were presented as the means ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (n ≥ 3 mice in each group). Normal, control group treated with 0.9% NaCl; IBD, murine model group treated with TBNS; MSCsPPcon, model group treated with control MSCsPP; MSCsPPsiIL‐22#2, model group treated with IL‐22‐knockdown MSCsPP.