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. 2023 Jan 31;20:20. doi: 10.1186/s12974-023-02708-x

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — TMEM119⁺/MHC-II⁺ EVs are significant increased 28 days following ET-1 injection. A MHC-II IHC in selected regions of ET-1 and saline injected animals: injection site, adjacent corpus callosum (CC) and contralateral striatum. Scale bar indicates 100 µm. B Images taken at × 40 magnification depicting microglia morphology within stroke region, scale bar indicates 25 μm C TMEM119⁺MHC-II⁺ labelled events/μL of plasma from baseline and 28-day samples of saline injection group. D TMEM119⁺MHC-II⁺ labelled events/μL of plasma from baseline and 28-day samples of ET-1 injection group. E TMEM119⁺/MHC-II⁺ labelled events/µL of plasma from 28-day samples of ET-1 and saline injection groups. # Indicates statistical significance (p < 0.05) measured using Wilcoxon paired rank test. *Indicates statistical significance (p < 0.05) measured using Mann–Whitney test