TABLE 3.
Adhesins and binding proteins
Adhesin-ligand interaction | Gene | Location | Comments | Reference(s) |
---|---|---|---|---|
Protein-protein | ||||
CR2 (C3d binding proteins) | 194, 395, 563 | |||
60, 50 kDa | Hyphal surface (60 kDa), yeast cell membrane? (50 kDa) | Isolated whole cell hyphal extracts; binding species differ in glycosylation | 52, 60, 235, 289, 563 | |
55–60 kDa (minor 67–68, 20 kDa) | Hyphae | Hyphal extract, Western blot with antiserum to CR2 | 235 | |
66, 40, 20 kDa | Yeast cells, hyphae | Western blot of cell wall extract with antiserum to CR2; 40 kDa ubiquitinated | 300, 486 | |
94, 68, 60, 50, 31 kDa | Hyphae | Western blot of cell wall extract with antiserum to CR2 | 145 | |
iC3b binding proteins | Yeast cells, hyphae | Epithelial, endothelial cell adhesin | 25, 26, 115, 146, 162, 176, 194 | |
130 kDa (also 100, 50 kDa) | Hyphae | Immunoprecipitation of surface proteins by MAb OKM-1 | 117 | |
165 kDa | INT1? | Yeast cells | Western blot of yeast cell cytosol, membrane, but not cell wall extracts with MAb OKM-1 | 153, 210 |
66, 55, 42 kDa | Hyphae | C3 affinity chromatography of soluble hyphal cell extract; 42 kDa binds C3, C3b, iC3b; 55 kDa binds C3 >C3b, iC3b; 66 kDa no binding | 4 | |
Fibrinogen binding proteins | 34, 358, 548 | |||
mp58 (58 kDa) | FBP1 | Some yeast cells, hyphae | Fibrinogen binding; ubiquitinated, collagen-like domains | 5, 60, 484, 486 |
68, 60–62 kDa | Hyphae | Multifunctional binding; fibrinogen, plastic, laminin, C3d | 33–35, 550 | |
Laminin binding proteins | ||||
p37 (37 kDa), 67 kDa | Yeast cell, hyphal cell wall | 67 kDa only yeast cell wall; p37 of yeast but not hyphal cells binds laminin; reacts with human high-affinity receptor Ab; p37 ubiquitinated, collagen-like domains | 296, 484, 486 | |
68, 62, 60 kDa | Hyphae > yeast cells | Multifunctional binding: fibrinogen, plastic, laminin, C3d | 33–35 | |
Multiple species | Ligand affinity blot of yeast cell wall extract | 164 | ||
Fibronectin binding protein | Differences reported for fibronectin binding site | 222, 248, 253, 257, 380, 407, 466, 507 | ||
62, 72 kDa (60, 105 kDa unreduced) | Yeast cells, hyphae | Affinity chromatography gelatin or fibronectin; 60-kDa species reacts with MAb OKM-1; 60-kDa species highly glycosylated; more species identified Ab to human fibronectin receptor | 256, 259 | |
Multiple species | Yeast cells | Yeast cell wall extract; anti-human fibronectin receptor antibody three proteins 40–50 kDa, polydisperse >143; ligand affinity blot additional proteins | 164 | |
Collagen binding proteins | 249, 255, 259 | |||
62, 72 kDa (60, 105 kDa unreduced) | Yeast cells, hyphae | Affinity chromatography gelatin or fibronectin; 60-kDa species reacts with MAb OKM-1 | 259 | |
Entactin binding proteins | ||||
65, 44, 25 kDa | Yeast cells, hyphae | Ligand affinity blot; RGD dependent and independent | 293 | |
Vitronectin binding protein | ||||
30 kDa | Yeast cells | Ligand affinity blot with cell wall extract | 222, 286, 505 | |
Ala1p | ALA1 | ? | Confers collagen IV, laminin and fibronectin binding on S. cerevisiae, member agglutinin-like gene family | 154a |
Protein-sugar | ||||
Fucose binding protein | Yeast cells, hyphae? | Buccal, vaginal epithelial cell adhesin; strain variation | 37, 53, 92, 93, 345, 544 | |
>15.7 kDa | 53, 544 | |||
GlcNAc binding protein | Yeast cells, hyphae? | Buccal, vaginal epithelial cell adhesin; strain variation | 37, 53, 92, 93, 345, 544 | |
190 kDa | Yeast cells | Cell wall extracts of galactose- but not glucose-grown cells; not known if is the same protein in reference 345 | 129 | |
Protein-glycosphingolipid | ||||
Fimbrial protein 66 kDa? | Yeast cells, hyphae? | Epithelial cell adhesin; adhesintope shared with P. aeruginosa pili | 279, 583–586 | |
Protein-presumed protein | ||||
Secreted aspartyl proteinase, 42–45 kDa |
SAP1-9 | Yeast cells, hyphae | Binding endothelial cells, corneocytes | 147, 433; Table 1 |
Protein-plastic | ||||
Plastic binding proteins | Binding to many plastics, environmental influences | 181, 243, 344, 458 | ||
60, 68, 200, >200 kDa | Hyphae | 60, 68 kDa may be multifunctional binding fibrinogen, laminin, C3d | 33, 34, 549 | |
30 kDa | AAF1 | Yeast cells, hyphae | Bind polystyrene and epithelial cells | 20, 21 |
Carbohydrate-protein | ||||
Acid labile mannan | ||||
β-1,2 mannotetraose | Yeast cells, hyphae | Mannose receptor, splenic, lymph node macrophage | 73, 285 | |
Carbohydrate-unknown | ||||
Acid-stable mannan | ||||
Factor 6 | Yeast cells, hyphae | BEC adhesion | 357 | |
Unknown | Yeast cells, hyphae | Splenic, lymph node macrophage | 234 |