Figure 2 ∣. Coordination of AMIO in rCa_v1.1.

(A) AMIO inserts into the fenestration on the interface of repeats III and IV (the III-IV fenestration). The PD of rCa_v1.1A is shown as ribbon cartoon (left) or semi-transparent surface (right) to highlight the binding pocket for AMIO. (B) AMIO occupies the same binding site as the DHP compounds. Structures of the α1 subunit of rCa_v1.1A and nifedipine (cyan sticks)-bound rCa_v1.1 (colored wheat, PDB code: 6JP5) can be superimposed with RMSD of 0.57 Å over 970 aligned Cα atoms. (C) AMIO is coordinated exclusively through van der Waals contacts. AMIO and the surrounding residues are shown as sticks. See also Figure S2.