Key Resources Table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and virus strains | ||
| E. coli BL21(DE3) | Novagen | Cat# 69387-1 |
| E. coli HST08 (Stellar™) | TaKaRa | Cat# 636766 |
| Biological samples | ||
| Rabbit muscle tissue | Pel-Freez | Cat# 41225 -2 |
| Chemicals, peptides, and recombinant proteins | ||
| Amiodarone | MedChemExpress | Cat# HY-14188 |
| MNI-1 | Merck & Co., Inc., Kenilworth, NJ, USA | N/A |
| Sofosbuvir | MedChemExpress | Cat# HY-15005 |
| Isradipine | Carbosynth Ltd | Cat# 12-35-21-00 |
| n-Dodecyl-β-D-Maltoside (DDM) | Anatrace | Cat# D310S |
| Glyco-diosgenin (GDN) | Anatrace | Cat# GDN101 |
| Cholesteryl hemisuccinate Tris salt (CHS) | Anatrace | Cat# CH210 |
| Aprotinin | VWR Life Science | Cat# 97062-754 |
| Pepstatin | VWR Life Science | Cat# 97064-248 |
| anti-Flag M2 affinity gel | MilliporeSigma | Cat# A2220 |
| Flag peptide | GenScript | Cat# RP10586-1 |
| Glutathione Sepharose® 4B | MilliporeSigma | Cat# GE17-0756-05 |
| Dulbecco’s modified Eagle Glutamax’s medium | Invitrogen | Cat# 10566-016 |
| Freestyle 293 medium | Thermo Fisher Scientific | Cat# 12338018 |
| Fetal bovine serum (influx assay) | Invitrogen | Cat# 10082-139 |
| Fetal Bovine Serum (protein expression) | Thermo Fisher Scientific | Cat# 10437028 |
| LB medium | Sigma | Cat# L3522 |
| Penicillin-streptomycin (100 U/mL) | Invitrogen | Cat# 15140-148 |
| Geneticin (G418) | Invitrogen | Cat# 10131-035 |
| Zeocin | Invitrogen | Cat# R250-01 |
| Hygromycin B | Invitrogen | Cat# 10687-010 |
| Polyethylenimine (PEI) | Polysciences | Cat# 24765-1 |
| Codex ACTOne® dye | Codex Biosolution Inc. | Cat#: CB-80599-301 |
| Superose 6™ Increase 10/300 GL | GE Healthcare | Cat# 29-0915-96 |
| Deposited data | ||
| Coordinates of rCav1.1A | This paper | PDB: 8E56 |
| Coordinates of rCav1.1AM0.1 | This paper | PDB: 8E57 |
| Coordinates of rCav1.1AM | This paper | PDB: 8E58 |
| Coordinates of hCav1.3A | This paper | PDB: 8E59 |
| Coordinates of hCav1.3S | This paper | PDB: 8E5A |
| Coordinates of hCav1.3AS | This paper | PDB: 8E5B |
| Cryo-EM map of rCav1.1A | This paper | EMDB: EMD-27904 |
| Cryo-EM map of rCav1.1AM0.1 | This paper | EMDB: EMD-27905 |
| Cryo-EM map of rCav1.1AM | This paper | EMDB: EMD-27906 |
| Cryo-EM map of hCav1.3A | This paper | EMDB: EMD-27907 |
| Cryo-EM map of hCav1.3S | This paper | EMDB: EMD-27908 |
| Cryo-EM map of hCav1.3AS | This paper | EMDB: EMD-27909 |
| Experimental models: Cell lines | ||
| HEK293F cells | Thermo Fisher Scientific | Cat# R79007 |
| HEK293 cells stably overexpressing human Cav1.2 channel complex and Kir2.3 | (Xia et al., 2004) | N/A |
| Recombinant DNA | ||
| Codon-optimized human Cav1.3-α1 cloned into a modified pCAG vector with tandem twin-strep and Flag tags at the amino (N) terminus | This paper | N/A |
| Codon-optimized human α2δ-1 subunit cloned into a modified pCAG vector | This paper | N/A |
| Codon-optimized human β3 subunit cloned into a modified pCAG vector with N-terminal Flag tag and C-terminal His10 tag | This paper | N/A |
| Software and algorithms | ||
| PyMOL | The PyMOL Molecular Graphics System, Schrodiner | https://pymol.org/2/ |
| Prism 9 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| MotionCor2 | (Zheng et al., 2017) | https://emcore.ucsf.edu/ucsf-motioncor2 |
| Coot | (Emsley et al., 2010) | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| UCSF Chimera | (Pettersen et al., 2004) | http://www.cgl.ucsf.edu/chimera |
| UCSF ChimeraX | (Goddard et al., 2018) | https://www.rbvi.ucsf.edu/chimerax/ |
| PHENIX | (Adams et al., 2010) | http://www.phenix-online.org/ |
| RELION 3.0 | (Zivanov et al., 2018) | https://www3.mrc-lmb.cam.ac.uk/relion/ |
| SerialEM | (Mastronarde, 2005) | http://bio3d.colorado.edu/SerialEM/ |
| Schrödinger Suite 2018-1 | Schrödinger, Inc. | https://www.schrodinger.com/ |